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Nicotine Test

Nicotine Test

SKU: DCT-102

The COT One Step Cotinine Test Device (Urine) is a lateral flow chromatographic immunoassay for the detection of Cotinine in human urine at a cut-off concentration of 200 ng/mL. Cotinine is a principle and residual metabolite of Nicotine which remains in the body of habitual tobacco users.

This test is intended for forensic, non-diagnostic use only to determine the smoking status and use of tobacco/nicotine only. This test is not intended to diagnose any medical condition or aliment. It is used for forensic use only.

Market price: $124.00 Our price: $114.00
Quantity 2-3 Items 4+ Items
Price $110.00 $102.00
Please note: Quantity of '1' means you are ordering 1 Box of 40 tests.. For example: Quantity of '3' means you are ordering 120 tests. Tests are sold in manufacturer supplied, original packaging.
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nicotine testing
 Integrated 5 Panel (COC/ mAMP/ THC/ OPI/ PCP) E-Z Test Cup II
Integrated 5 Panel (COC/ mAMP/ THC/ OPI/ PCP) E-Z Test Cup II

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Quantity (no.)
Discounted Price
1-24 $3.20 $2.45 each
25-49 $3.00 $2.35each
50-99 $2.80 $2.25 each
100-100+ $2.60 $2.15 each

The COT One Step Cotinine Test Device (Urine) is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Cotinine in urine. The COT One Step Cotinine Test Device (Urine) yields a positive result when the Cotinine in urine exceeds 200 ng/mL.

The following table lists compounds that are positively detected in urine by the COT
One Step Cotinine Test Device (Urine) at 5 minutes.

Compound Concentration (ng/mL)
(-)-Cotinine 200
(-)-Nicotine 6,250

About Cotinine

Cotinine is the first-stage metabolite of nicotine, a toxic alkaloid that produces stimulation of the autonomic ganglia and central nervous system when in humans. Nicotine is a drug to which virtually every member of a tobacco-smoking society is exposed whether through direct contact or second-hand inhalation. In addition to tobacco, nicotine is also commercially available as the active ingredient in smoking replacement therapies such as nicotine gum, transdermal patches and nasal sprays. In a 24-hour urine, approximately 5% of a nicotine dose is excreted as unchanged drug with 10% as cotinine and 35% as hydroxycotinine; the concentrations of other metabolites are believed to account for less than 5%.1 While cotinine is thought to be an inactive metabolite, it’s elimination profile is more stable than that of nicotine which is largely urine pH dependent. As a result, cotinine is considered a good biological marker for determining nicotine use. The plasma half-life of nicotine is approximately 60 minutes following inhalation or parenteral administration.2 Nicotine and cotinine are rapidly eliminated by the kidney; the window of detection for cotinine in urine at a cutoff level of 200 ng/mL is expected to be up to 2-3 days after nicotine use.

The COT One Step Cotinine Test Device (Urine) provides only a qualitative, preliminary analytical result. A secondary analytical method must be used to obtain a confirmed result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method.1 For more information, click here.